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A Fluorescence Switch for the Detection of Nitric Oxide and Histidine and Its Application in Live Cell Imaging
Author(s) -
Sivaraman Gandhi,
Anand Thangaraj,
Chellappa Duraisamy
Publication year - 2014
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201402217
Subject(s) - histidine , fluorescence , chemistry , nitric oxide , rhodamine , photochemistry , live cell imaging , amino acid , cell , biochemistry , organic chemistry , optics , physics
A rhodamine‐based copper complex as a selective, sensitive turn‐on fluorescent chemosensor for NO/histidine has been developed. A conspicuous fluorescence enhancement was observed in the presence of nitric oxide/histidine. The probe was specific towards NO over other reactive oxygen species and reactive nitrogen species. The probe showed selective fluorescence enhancement with histidine; the other naturally occurring amino acids did not result in fluorescence enhancement. EPR and ESIMS studies clearly showed that NO‐induced reduction of copper ions leads to the fluorescence enhancement. The viability of the probe for fluorescent imaging of nitric oxide and histidine in living cells has been demonstrated by means of confocal laser scanning microscopy experiments.