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Titanium(IV)‐Immobilized Hydrophilic Hierarchically Ordered Macro‐/Mesoporous Silica for Fast Enrichment of Phosphopeptides
Author(s) -
Yan Yinghua,
Zheng Zhifang,
Li Yan,
Deng Chunhui,
Zhang Xiangmin
Publication year - 2014
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201300411
Subject(s) - selectivity , mesoporous material , titanium , mesoporous silica , nanomaterials , materials science , coating , chemistry , combinatorial chemistry , molar ratio , nanocomposite , chemical engineering , nanotechnology , catalysis , organic chemistry , engineering
Feasible design is essential to achieve ideal chemical and biological properties of nanomaterials. For the first time, new nanocomposites with a polydopamine coating on hierarchically ordered macro‐/mesoporous silica functionalized with titanium ions (denoted as HOMMS‐PD‐Ti 4+ ) were prepared through a facile reaction route at room temperature. The applicability of as‐synthesized HOMMS‐PD‐Ti 4+ for the selective enrichment of phosphopeptides was tested. The experimental results demonstrate that, by taking advantage of the pure phosphate–Ti 4+ interface and high loading amount of Ti 4+ , HOMMS‐PD‐Ti 4+ presents remarkable selectivity for phosphopeptides, even at a low molar ratio of phosphopeptides/non‐phosphopeptides (1:1000) with a very rapid enrichment speed (within 1 min). The superior sensitivity for low‐abundant phosphopeptides and the high selectivity and effectiveness for the enrichment of phosphopeptides from human serum are also proven. These outstanding features demonstrate that HOMMS‐PD‐Ti 4+ exhibits great potential in phosphoproteome research in the future.