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Understanding the Photochemical Pathway of In Vitro Target Delivery of Aluminium Phthalocyanine: A Mechanistic Approach Using Radical Reaction Chemistry
Author(s) -
Akpe Victor,
Vernet Erik,
Madu Christian,
Obirai Joseph C.,
Brismar Hjalmar
Publication year - 2014
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201300402
Subject(s) - photobleaching , photochemistry , chemistry , fluorescence , chromophore , conjugated system , phthalocyanine , phosphorescence , singlet oxygen , radical , organic chemistry , polymer , physics , quantum mechanics , oxygen
A classical dye, aluminium phthalocyanine (AlPc), is used to study the photochemical processes involved in the chromophore‐assisted laser inactivation technique. Both cell‐free and cell‐based systems are investigated by novel methods and radical reaction chemistry. Findings on the photochemical pathways in two models representing cell‐free and a cell‐based systems are reported. In the cell‐free system, the unsubstituted, free, fluorescence‐active photosensitiser AlPc recovers its fluorescence signal by means of phosphorescence through a reversible photobleaching process. In the cell‐based system, photoactivation of substituted AlPc conjugated to an antibody results in the loss of fluorescence signal at the area examined. Reinjection of the AlPc‐conjugated antibodies restores the fluorescence signal.