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Synthesis of C 8 ‐Functionalized Magnetic Graphene with a Polydopamine Coating for the Enrichment of Low‐Abundance Peptides
Author(s) -
Zhao Man,
Deng Chunhui,
Zhang Xiangmin
Publication year - 2014
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201300362
Subject(s) - chemistry , silanization , coating , graphene , polymerization , chromatography , combinatorial chemistry , chemical engineering , nuclear chemistry , nanotechnology , organic chemistry , polymer , materials science , engineering
For the first time, C 8 ‐functionalized magnetic graphene (magG) has been designed and synthesized with a polydopamine (PDA) coating. The magG prepared by a solvothermal reaction was encapsulated in a layer of PDA through the oxidative polymerization of dopamine in alkaline buffer, and C 8 groups were grafted onto magG/PDA composites through a silanization method. The as‐prepared material integrates the merits of graphene, magnetic microspheres, PDA, and C 8 chains; thus possessing an ultrahigh specific area, strong magnetic responsiveness, excellent solubility, and an extraordinary enrichment capability. The C 8 ‐functionalized magnetic composites were employed in the enrichment and identification of low‐concentration standard peptides, peptides in standard protein digest solutions, and endogenous peptides in human urine. The enriched peptides were eluted and analyzed by MALDI‐TOF MS. The MS results indicated that the C 8 ‐functionalized material exhibited the distinguished ability to enrich hydrophobic peptides mainly through hydrophobic–hydrophobic interactions. Moreover, thanks to the enrichment approach based on magG@PDA@C 8 , the limit of detection of the standard peptide decreased to as low as 50 pM. The experimental results demonstrate that the magG@PDA@C 8 composite is a promising candidate for the enrichment of low‐abundance peptides in biological samples.