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Derivatization and Multidimensional Gas‐Chromatographic Resolution of α‐Alkyl and α‐Dialkyl Amino Acid Enantiomers
Author(s) -
Meinert Cornelia,
Meierhenrich Uwe J.
Publication year - 2014
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201300328
Subject(s) - enantioselective synthesis , chemistry , enantiomer , derivatization , amino acid , homochirality , alkyl , organic chemistry , resolution (logic) , chromatography , combinatorial chemistry , high performance liquid chromatography , catalysis , artificial intelligence , computer science , biochemistry
The quantitative resolution of amino acid enantiomers is crucial to a number of fields of bioanalytical chemistry, including food and plant analysis, metabolomics, medicinal chemistry, and archeochemistry. The enantioselective analysis of α‐dialkyl amino acids, however, has proven more difficult than for proteinaceous α‐protonated amino acids. Herein, the development of an analytical method capable of base‐line resolving enantiomers of α‐alkyl and α‐dialkyl amino acids in two chromatographic dimensions is reported. It was found that enantiomers of α‐dialkyl amino acids can be readily resolved with the use of an enantioselective Chirasil‐Val stationary phase in the first chromatographic dimension and a polyethylene glycol stationary phase in the second dimension. These data offer new possibilities for the enantioselective resolution and precise quantification of non‐biological α‐dialkyl amino acids. This methodology will subsequently be applied particularly towards enantioselective analyses of samples relevant for understanding of the origin of homochirality on Earth such as meteorites, comets, and interstellar ices.