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The Binding of a Hydroxy‐9,10‐anthraquinone Cu II Complex to Calf Thymus DNA: Electrochemistry and UV/Vis Spectroscopy
Author(s) -
Guin Partha S.,
Mandal Parikshit C.,
Das Saurabh
Publication year - 2012
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201100046
Subject(s) - binding constant , cyclic voltammetry , anthraquinone , dna , anthraquinones , fluorescence spectroscopy , chemistry , spectroscopy , quinone , combinatorial chemistry , fluorescence , anthracycline , molecule , photochemistry , ultraviolet visible spectroscopy , stereochemistry , biophysics , binding site , electrochemistry , organic chemistry , biochemistry , biology , cancer , botany , physics , electrode , quantum mechanics , breast cancer , genetics
Anthracyclines are broad‐spectrum antibiotics that are used against several human cancers. Hydroxy‐9,10‐anthraquinone compounds closely resemble anthracycline antibiotics from a structural and functional viewpoint. High cost and aspects of toxicity introduce limitations on its use. On complex formation the toxicities of anthracyclines decrease. A close analogue is sodium 1,4‐dihydroxy‐9,10‐anthraquinone‐2‐sulphonate (AS), which is a comparatively cheaper molecule and resembles a good number of anthracycline derivatives. It forms a complex with Cu II , [Cu(AS) 2 ]. The complex interacts with calf thymus DNA that was studied by UV/Vis spectroscopy and cyclic voltammetry. The experimental data obtained from the two techniques was analyzed for binding constant and size of the binding site. These results not only corroborate each other but also justify earlier data obtained using fluorescence spectroscopy. An important aspect to this study was that nonlinear curve fitting, which is usually applied for analyzing DNA interaction using fluorescence and absorption spectroscopy, was used for the first time to analyze the interaction of the complex with DNA using cyclic voltammetry by monitoring the change in the reduction peak current ( I p ). The DNA binding data provides an insight into a probable mode of action of the hydroxy‐9,10‐anthraquinones within cells as modification of DNA that leads to cell death is an important aspect in cancer research.