Open AccessCharacterization and Purification of Mouse Mucosal‐Associated Invariant T (MAIT) Cells
Author(s) -
Chen Zhenjun,
Wang Huimeng,
D'Souza Criselle,
Koay HuiFern,
Meehan Bronwyn,
Zhao Zhe,
Pediongco Troi,
Shi Mai,
Zhu Tianyuan,
Wang Bingjie,
KjerNielsen Lars,
Eckle Sidonia B. G.,
Rossjohn Jamie,
Fairlie David P.,
Godfrey Dale I.,
Strugnell Richard A.,
McCluskey James,
Corbett Alexandra J.
Publication year - 2019
Publication title -
current protocols in immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 43
eISSN - 1934-368X
pISSN - 1934-3671
DOI - 10.1002/cpim.89
Subject(s) - invariant (physics) , chemistry , microbiology and biotechnology , biology , immunology , computational biology , mathematics , mathematical physics
This unit describes the utility of various mouse models of infection and immunization for studying mucosal‐associated invariant T (MAIT) cell immunity: MAIT cells can be isolated from the lungs (or from other tissues/organs) and then identified and characterized by flow cytometry using MR1 tetramers in combination with a range of antibodies. The response kinetics, cytokine profiles, and functional differentiation of lung MAIT cells are studied following infection with the bacterial pathogen Legionella longbeachae or Salmonella enterica Typhimurium or immunization with synthetic MAIT cell antigen plus Toll‐like receptor agonist. MAIT cells enriched or expanded during the process can be used for further studies. A step‐by‐step protocol is provided for MAIT cell sorting and adoptive transfer. Mice can then be challenged and MAIT cells tracked and further examined. © 2019 by John Wiley & Sons, Inc.