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Digital Imaging‐based Colourimetry for Enzymatic Processes in Transparent Liquid Marbles
Author(s) -
Nguyen NhatKhuong,
Singha Pradip,
Zhang Jun,
Phan HoangPhuong,
Nguyen NamTrung,
Ooi Chin Hong
Publication year - 2021
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.202000760
Subject(s) - microreactor , absorbance , starch , chemistry , repeatability , enzymatic hydrolysis , hydrolysis , linear range , chromatography , organic chemistry , catalysis , detection limit
Liquid marbles are a promising microreactor platform that recently attracts significant research interest owing to their ability to accommodate a wide range of micro reactions. However, the use of destructive and ex‐situ methods to monitor reactions impairs the potential of liquid‐marble‐based microreactors. This paper proposes a non‐destructive, in situ, and cost‐effective digital‐imaging‐based colourimetric monitoring method for transparent liquid marbles, using the enzymatic hydrolysis of starch as an illustrative example. The colourimetric reaction between starch and iodine produces a complex that exhibits a dark blue colour. We found that the absorbance of red channel of digital images showed a linear relationship with starch concentration with high sensitivity and repeatability. This digital‐imaging‐based colourimetric method was used to study the hydrolysis of starch by α‐amylase. The results show high accuracy and applicability of first‐order kinetics for this reaction. The demonstration of digital‐imaging‐based colourimetry indicates the potential of liquid marble‐based microreactors.