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Cover Feature: The Photocatalyzed Thiol‐ene reaction: A New Tag to Yield Fast, Selective and reversible Paramagnetic Tagging of Proteins (ChemPhysChem 9/2020)
Author(s) -
Denis Maxime,
Softley Charlotte,
Giuntini Stefano,
Gentili Matteo,
Ravera Enrico,
Parigi Giacomo,
Fragai Marco,
Popowicz Grzegorz,
Sattler Michael,
Luchinat Claudio,
Cerofolini Linda,
Nativi Cristina
Publication year - 2020
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.202000286
Subject(s) - paramagnetism , chemistry , ene reaction , alkene , thiol , cysteine , yield (engineering) , photochemistry , stereochemistry , organic chemistry , materials science , catalysis , physics , quantum mechanics , enzyme , metallurgy
The Cover Feature illustrates the photo‐initialization by UV light of the thiol‐ene reaction between the alkene of a newly designed paramagnetic tag and the thiol group of the cysteine residue on a model protein. The protein tagging occurs in 1 hour. We tagged the protein with three different lanthanide ions, which induced different chemical shifts on the protein NMR signals. Paramagnetic restraints were measured and the tensor calculated. More information can be found in the Article by L. Cerofolini, C. Nativi and co‐workers.