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Rapid Detection of AIB1 in Breast Cancer Cells Based on Aptamer‐Functionalized Nanomotors
Author(s) -
BeltránGastélum Mara,
EstebanFernández de Ávila Berta,
Gong Hua,
Venugopalan Pooyath Lekshmy,
Hianik Tibor,
Wang Joseph,
Subjakova Veronika
Publication year - 2019
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201900844
Subject(s) - aptamer , graphene , fluorescence , nanotechnology , intracellular , fluorescein , breast cancer , molecular beacon , biophysics , chemistry , dna , materials science , cancer , cancer research , biochemistry , microbiology and biotechnology , oligonucleotide , medicine , biology , physics , quantum mechanics
Herein, we report ultrasound‐propelled graphene‐oxide coated gold nanowire motors, functionalized with fluorescein‐labeled DNA aptamers (FAM‐AIB1‐apt), for qualitative detection of overexpressed AIB1 oncoproteins in MCF‐7 breast cancer cells. The movement of nanomotors under the ultrasound field facilitated intracellular uptake and resulted in a faster aptamer binding with the target protein and thus faster fluorescence recovery. The propulsion behavior of the aptamer functionalized nanomotors greatly enhanced the fluorescence intensity compared to static conditions. The new aptamer@nanomotor‐based strategy offers considerable potential for further development of sensing methodologies towards diagnosis of breast cancer.