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DNA Aptamers in the Detection of Leukemia Cells by the Thickness Shear Mode Acoustics Method
Author(s) -
Poturnayová Alexandra,
Buríková Monika,
Bízik Jozef,
Hianik Tibor
Publication year - 2019
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201801126
Subject(s) - aptamer , jurkat cells , force spectroscopy , biosensor , leukemia , receptor , myeloid leukemia , cell , chemistry , dna , biophysics , cell membrane , cell surface receptor , microbiology and biotechnology , biology , materials science , nanotechnology , cancer research , biochemistry , immunology , atomic force microscopy , immune system , t cell
By using the thickness shear mode acoustics method (TSM) and single‐molecule force spectroscopy (SMFS) we studied the interactions between DNA aptamers (sgc8c) specific to the protein tyrosine kinase 7 (PTK7), which is localized in the membranes of leukemia lymphoblastics (MOLT‐4), and lymphocyte (Jurkat) cell lines, as well with PTK7‐negative U266 myeloid leukemia cells. The TSM method allowed the development of a highly sensitive, label‐free biosensor for the detection leukemia cells with a limit of detection of (195±20) cells/mL. SMFS approved the high selectivity of the sgc8c aptamers to the PTK7 receptors at the cell surface and allowed determining the binding probability of the aptamers to the PTK7 receptors at different cell lines.