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Metabolic Studies of Tumor Cells Using [1‐ 13 C] Pyruvate Hyperpolarized by Means of PHIP‐Side Arm Hydrogenation
Author(s) -
Cavallari Eleonora,
Carrera Carla,
Aime Silvio,
Reineri Francesca
Publication year - 2019
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201800652
Subject(s) - hyperpolarization (physics) , chemistry , glycolysis , metabolic pathway , pkm2 , kinetics , pyruvic acid , lactate dehydrogenase , biochemistry , biophysics , metabolism , pyruvate kinase , enzyme , stereochemistry , nuclear magnetic resonance spectroscopy , biology , physics , quantum mechanics
Abstract The kinetics of metabolic processes can be assessed, in real time by means of MR hyperpolarized (HP) metabolites. [1‐ 13 C]pyruvate, hyperpolarized by means of d‐DNP, is, by far, the substrate most widely applied to the investigation of several pathologies characterized by deregulated glycolytic metabolic networks, including cancer. Hyperpolarization of [1‐ 13 C]pyruvate by means of the cost effective, fast and easy to handle PHIP‐SAH (para‐hydrogen induced polarization‐side arm hydrogenation) method opens‐up a pathway for the application of HP metabolites to a wide range of cancer‐related studies. Herein, we report the first application of PHIP‐SAH hyperpolarized [1‐ 13 C]pyruvate in the investigation of upregulated glycolysis in two murine breast cancer cell lines (168FARN and 4T1). The results obtained using HP pyruvate have been validated with a conventional biochemical assay and are coherent with previously‐reported lactate dehydrogenase activity measured in those cells.