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Modulation of Solvation and Molecular Recognition of a Lipid Bilayer under Dynamical Phase Transition
Author(s) -
Singh Priya,
Choudhury Susobhan,
Sharma V. K.,
Mitra S.,
Mukhopadhyay R.,
Das Ranjan,
Pal Samir Kumar
Publication year - 2018
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201800522
Subject(s) - lipid bilayer , chemistry , solvation , lipid bilayer phase behavior , bilayer , förster resonance energy transfer , vesicle , chemical physics , phase transition , molecular dynamics , biophysics , molecule , fluorescence , membrane , organic chemistry , computational chemistry , biochemistry , physics , quantum mechanics , biology
It is well accepted in contemporary biology that an ∼30 Å thick lipid bilayer film around living cells is a matter of life and death as the film typically delimits the environments that serve as a crucial margin. The dynamic organization of lipid molecules both across the lipid bilayer and in the lateral dimension are known to be crucial for cellular transport and molecular recognition by important biological macromolecules. Here, we study dilute (20 mM) Dioctadecyldimethylammonium bromide (DODAB) vesicles at different temperatures in aqueous dispersion with well‐defined phases namely liquid crystalline, gel and subgel. The spectroscopic studies on two fluorescent probes 8‐anilino‐1‐naphthalene sulfonic acid ammonium salt (ANS) and Coumarin 500 (C500), former in the head group region of the lipid‐water interface and later located deeper in the lipid bilayer follow dynamics (solvation and fluidity) of their local environments in the vesicles. Binding of an anti‐tuberculosis drug rifampicin has also been studied employing Förster resonance energy transfer (FRET) technique. The molecular insight concerning the effect of dynamical organization of the lipid molecules on the local dynamics of aqueous environments in different phases leading to molecular recognition becomes evident in our study.