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Photon Yield Enhancement of Red Fluorophores at Cryogenic Temperatures
Author(s) -
Hulleman Christiaan N.,
Li Weixing,
Gregor Ingo,
Rieger Bernd,
Enderlein Jörg
Publication year - 2018
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201800131
Subject(s) - photobleaching , liquid nitrogen , microscopy , fluorescence , photon , yield (engineering) , resolution (logic) , materials science , two photon excitation microscopy , molecule , photoactivated localization microscopy , super resolution microscopy , chemistry , fluorescence microscope , analytical chemistry (journal) , optics , physics , organic chemistry , chromatography , artificial intelligence , computer science , metallurgy
Single Molecule Localization Microscopy has become one of the most successful and widely applied methods of Super‐resolution Fluorescence Microscopy. Its achievable resolution strongly depends on the number of detectable photons from a single molecule until photobleaching. By cooling a sample from room temperature down to liquid nitrogen temperatures, the photostability of dyes can be enhanced by more than 100 fold, which results in an improvement in localization precision greater than 10 times. Here, we investigate a variety of fluorescent dyes in the red spectral region, and we find an average photon yield between 3.5 ⋅ 10 6 to 11 ⋅ 10 6 photons before bleaching at liquid nitrogen temperatures, corresponding to a theoretical localization precision around 0.1 nm.