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Electrochemical Measurement of Dopamine Release and Uptake in Zebrafish Following Treatment with Carboplatin
Author(s) -
Field Thomas M.,
Shin Mimi,
Stucky Chase S.,
Loomis Joseph,
Johnson Michael A.
Publication year - 2018
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201701357
Subject(s) - carboplatin , dopamine , zebrafish , neurotransmitter , reuptake , pharmacology , medicine , chemistry , anesthesia , endocrinology , chemotherapy , central nervous system , biochemistry , serotonin , receptor , cisplatin , gene
Post‐chemotherapy cognitive impairment, also known as ‘chemobrain,’ is a neurological condition in which cognitive function is impaired as a result of cancer chemotherapy treatment. In this work, we used fast‐scan cyclic voltammetry (FSCV) to measure electrically evoked dopamine release and uptake in whole brain preparations from zebrafish that have been treated with carboplatin, an agent associated with chemobrain. We administered carboplatin by addition to the fish's tank water or their food. One week of treatment with 100 μM carboplatin in the water was needed to significantly impair dopamine release (∼40 % of control); however, only one day of treatment through the zebrafish's food was needed to cause a similar impairment. Atomic absorption spectroscopy measurements suggested that administration through food resulted in higher initial levels of carboplatin compared to water administration, but water administration resulted in an increase over time. Uptake, determined by modeling stimulated release plots, was unaffected. These results are consistent with our previous findings of diminished neurotransmitter release in rats and support a role for zebrafish in chemobrain‐related studies.