Premium
Double‐Strand Breaks in Genome‐Sized DNA Caused by Ultrasound
Author(s) -
Kubota Rinko,
Yamashita Yusuke,
Kenmotsu Takahiro,
Yoshikawa Yuko,
Yoshida Kenji,
Watanabe Yoshiaki,
Imanaka Tadayuki,
Yoshikawa Kenichi
Publication year - 2017
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201601325
Subject(s) - sonication , pulse (music) , pulse duration , dna , breakage , fluorescence , ultrasound , biophysics , chemistry , fluorescence microscope , materials science , analytical chemistry (journal) , optics , physics , laser , biology , biochemistry , chromatography , detector , acoustics , composite material
DNA double‐strand breaks (DSBs) caused by ultrasound were evaluated in a quantitative manner by single‐molecule fluorescence microscopy. We compared the effect of time‐interval (or pulse) sonication to that of continuous wave (CW) sonication at a fixed frequency of 30 kHz. Pulses caused fewer DSBs than CW sonication under the same total input ultrasound energy when the pulse repetition period was above the order of a second. In contrast, pulses caused more DSBs than CW sonication for pulse widths shorter than a second. These effect of ultrasound on DNA were interpreted in terms of the time‐dependent decay in the probability of breakage during the duration of a pulse. We propose a simple phenomenological model by considering a characteristic decay in the probability of DSBs during single‐pulse sonication, which reproduces the essence of the experimental trend. In addition, a data analysis revealed a characteristic scaling behavior between the number of pulses and the number of DSBs.