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Cisplatin Primary Complex with l ‐Histidine Target Revealed by IR Multiple Photon Dissociation (IRMPD) Spectroscopy
Author(s) -
Corinti Davide,
De Petris Alberto,
Coletti Cecilia,
Re Nazzareno,
Chiavarino Barbara,
Crestoni Maria E.,
Fornarini Simonetta
Publication year - 2017
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201601172
Subject(s) - infrared multiphoton dissociation , chemistry , imidazole , tandem mass spectrometry , electrospray ionization , spectroscopy , dissociation (chemistry) , mass spectrometry , infrared spectroscopy , histidine , photochemistry , fragmentation (computing) , stereochemistry , amino acid , organic chemistry , chromatography , biochemistry , physics , quantum mechanics , computer science , operating system
The primary complex obtained from cisplatin and l ‐histidine in water has been detected and isolated by electrospray ionization. The so‐obtained cis ‐[PtCl(NH 3 ) 2 (histidine)] + complex has been characterized in detail by high‐resolution mass spectrometry (MS), tandem MS, IR multiple photon dissociation (IRMPD) spectroscopy, and by quantum chemical calculations. The structural features revealed by IRMPD spectroscopy indicate that platinum binds to the imidazole group, which presents tautomeric forms. Thus, depending on the position of the amino acid pendant on the imidazole ring, isomeric complexes are formed that are remarkably different with respect to the ease with which they undergo fragmentation when activated either by energetic collisions or by multiple IR photon absorption. It is shown here how IRMPD kinetics can allow their relative proportions to be estimated.