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An Enzyme‐based 1:2 Demultiplexer Interfaced with an Electrochemical Actuator
Author(s) -
Fratto Brian E.,
Guz Nataliia,
Fallon Tyler T.,
Katz Evgeny
Publication year - 2017
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201600799
Subject(s) - demultiplexer , modular design , logic gate , hexokinase , signal (programming language) , chemistry , channel (broadcasting) , actuator , multiplexing , enzyme , computer science , nanotechnology , materials science , biochemistry , electronic engineering , engineering , algorithm , computer network , programming language , multiplexer , glycolysis , artificial intelligence , operating system
Abstract An enzyme‐based 1:2 demultiplexer is designed in a flow system composed of three cells where each one is modified with a different enzyme: hexokinase, glucose dehydrogenase and glucose‐6‐phosphate dehydrogenase. The Input signal activating the biocatalytic cascade is represented by glucose, while the Address signal represented by ATP is responsible for directing the Input signal to one of the output channels, depending on the logic value of the Address . The biomolecular 1:2 demultiplexer is extended to include two electrochemical actuators releasing entrapped DNA molecules in the active output channel. The modular design of the system allows for easy exchange and extension of the functional elements. The present demultiplexer can be easily integrated in various biomolecular logic systems, including different logic gates based on the enzyme‐ or DNA‐based reactions, as well as containing different chemical actuators, for example, with a biomolecular release function.