Premium
Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging
Author(s) -
Quérard Jérôme,
Le Saux Thomas,
Gautier Arnaud,
Alcor Damien,
Croquette Vincent,
Lemarchand Annie,
Gosse Charlie,
Jullien Ludovic
Publication year - 2016
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201500987
Subject(s) - analyte , biological system , kinetics , discriminative model , multiplexing , computer science , chemical kinetics , nanotechnology , chemistry , signature (topology) , singularity , topology (electrical circuits) , materials science , physics , artificial intelligence , mathematics , telecommunications , mathematical analysis , geometry , quantum mechanics , combinatorics , biology
Living cells are chemical mixtures of exceptional interest and significance, whose investigation requires the development of powerful analytical tools fulfilling the demanding constraints resulting from their singular features. In particular, multiplexed observation of a large number of molecular targets with high spatiotemporal resolution appears highly desirable. One attractive road to address this analytical challenge relies on engaging the targets in reactions and exploiting the rich kinetic signature of the resulting reactive module, which originates from its topology and its rate constants. This review explores the various facets of this promising strategy. We first emphasize the singularity of the content of a living cell as a chemical mixture and suggest that its multiplexed observation is significant and timely. Then, we show that exploiting the kinetics of analytical processes is relevant to selectively detect a given analyte: upon perturbing the system, the kinetic window associated to response read‐out has to be matched with that of the targeted reactive module. Eventually, we introduce the state‐of‐the‐art of cell imaging exploiting protocols based on reaction kinetics and draw some promising perspectives.