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Cover Picture: Two‐Color RESOLFT Nanoscopy with Green and Red Fluorescent Photochromic Proteins (ChemPhysChem 4/2014)
Author(s) -
LavoieCardinal Flavie,
Jensen Nickels A.,
Westphal Volker,
Stiel Andre C.,
Chmyrov Andriy,
Bierwagen Jakob,
Testa Ilaria,
Jakobs Stefan,
Hell Stefan W.
Publication year - 2014
Publication title -
chemphyschem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201490015
Subject(s) - photochromism , fluorescence , superresolution , cover (algebra) , chemistry , biophysics , resolution (logic) , photochemistry , optics , biology , physics , image (mathematics) , computer science , mechanical engineering , artificial intelligence , engineering
The cover image shows the switching scheme used by S. Jakobs, S. W. Hell et al. in two‐color RESOLFT imaging. Green‐ and red‐emitting reversible switchable fluorescent proteins (RSFPs) are fused to cytoskeletal proteins and expressed in human cells. The RSFPs in the periphery of the excitation focus are sequentially switched off and the fluorescence of the proteins in the on‐state is detected. The combination of green‐ and red‐emitting RSFPs provides two‐color imaging of whole mammalian cells with a spatial resolution up to four times beyond the diffraction barrier, as shown on p. 655 .