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Dark‐Field‐Based Observation of Single‐Nanoparticle Dynamics on a Supported Lipid Bilayer for In Situ Analysis of Interacting Molecules and Nanoparticles
Author(s) -
Lee Young Kwang,
Kim Sungi,
Nam JwaMin
Publication year - 2015
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201402529
Subject(s) - nanoparticle , bilayer , nanotechnology , context (archaeology) , lipid bilayer , plasmonic nanoparticles , materials science , plasmon , molecule , chemical physics , molecular dynamics , chemistry , optoelectronics , membrane , computational chemistry , paleontology , biochemistry , organic chemistry , biology
Observation of single plasmonic nanoparticles in reconstituted biological systems allows us to obtain snapshots of dynamic processes between molecules and nanoparticles with unprecedented spatiotemporal resolution and single‐molecule/single‐particle‐level data acquisition. This Concept is intended to introduce nanoparticle‐tethered supported lipid bilayer platforms that allow for the dynamic confinement of nanoparticles on a two‐dimensional fluidic surface. The dark‐field‐based long‐term, stable, real‐time observation of freely diffusing plasmonic nanoparticles on a lipid bilayer enables one to extract a broad range of information about interparticle and molecular interactions throughout the entire reaction period. Herein, we highlight important developments in this context to provide ideas on how molecular interactions can be interpreted by monitoring dynamic behaviors and optical signals of laterally mobile nanoparticles.