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Fluorescence Microscopy with 6 nm Resolution on DNA Origami
Author(s) -
Raab Mario,
Schmied Jürgen J.,
Jusuk Ija,
Forthmann Carsten,
Tinnefeld Philip
Publication year - 2014
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201402179
Subject(s) - dna origami , microscopy , superresolution , nanoscopic scale , fluorescence microscope , resolution (logic) , super resolution microscopy , image resolution , photoactivated localization microscopy , point spread function , monte carlo method , materials science , nanotechnology , optics , fluorescence , nanostructure , physics , computer science , computer vision , artificial intelligence , image (mathematics) , statistics , mathematics
Resolution of emerging superresolution microscopy is commonly characterized by the width of a point‐spread‐function or by the localization accuracy of single molecules. In contrast, resolution is defined as the ability to separate two objects. Recently, DNA origamis have been proven as valuable scaffold for self‐assembled nanorulers in superresolution microscopy. Here, we use DNA origami nanorulers to overcome the discrepancy of localizing single objects and separating two objects by resolving two docking sites at distances of 18, 12, and 6 nm by using the superresolution technique DNA PAINT(point accumulation for imaging in nanoscale topography). For the smallest distances, we reveal the influence of localization noise on the yield of resolvable structures that we rationalize by Monte Carlo simulations.