Premium
Far‐Red Organic Fluorophores Contain a Fluorescent Impurity
Author(s) -
Stone Matthew B.,
Veatch Sarah L.
Publication year - 2014
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201402002
Subject(s) - fluorescence , microscopy , impurity , fluorescence microscope , super resolution microscopy , resolution (logic) , materials science , optical microscope , chemistry , optics , nanotechnology , photochemistry , analytical chemistry (journal) , scanning electron microscope , physics , chromatography , organic chemistry , artificial intelligence , computer science
Far‐red organic fluorophores commonly used in traditional and super‐resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near‐red emission. This near‐red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super‐resolution localization microscopy.