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Spatially Selective Heteronuclear Multiple‐Quantum Coherence Spectroscopy for Biomolecular NMR Studies
Author(s) -
Sathyamoorthy Bharathwaj,
Parish David M.,
Montelione Gaetano T.,
Xiao Rong,
Szyperski Thomas
Publication year - 2014
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201301232
Subject(s) - heteronuclear molecule , chemistry , nuclear magnetic resonance spectroscopy , coherence (philosophical gambling strategy) , spectroscopy , heteronuclear single quantum coherence spectroscopy , isoleucine , protonation , valine , analytical chemistry (journal) , two dimensional nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , relaxation (psychology) , spectral line , crystallography , stereochemistry , leucine , amino acid , physics , organic chemistry , ion , biochemistry , quantum mechanics , social psychology , psychology , astronomy
Spatially selective heteronuclear multiple‐quantum coherence (SS HMQC) NMR spectroscopy is developed for solution studies of proteins. Due to “time‐staggered” acquisitioning of free induction decays (FIDs) in different slices, SS HMQC allows one to use long delays for longitudinal nuclear spin relaxation at high repetition rates. To also achieve high intrinsic sensitivity, SS HMQC is implemented by combining a single spatially selective 1 H excitation pulse with nonselective 1 H 180° pulses. High‐quality spectra were obtained within 66 s for a 7.6 kDa uniformly 13 C, 15 N‐labeled protein, and within 45 and 90 s for, respectively, two proteins with molecular weights of 7.5 and 43 kDa, which were uniformly 2 H, 13 C, 15 N‐labeled, except for having protonated methyl groups of isoleucine, leucine and valine residues.