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Fingerprinting DNA Oxidation Processes: IR Characterization of the 5‐Methyl‐2′‐Deoxycytidine Radical Cation
Author(s) -
Bucher Dominik B.,
Pilles Bert M.,
Pfaffeneder Toni,
Carell Thomas,
Zinth Wolfgang
Publication year - 2014
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201300954
Subject(s) - chemistry , cytidine , mass spectrometry , dna , photochemistry , radical ion , demethylation , dna oxidation , hydroxyl radical , dna damage , dna methylation , ion , organic chemistry , biochemistry , radical , chromatography , gene , gene expression , enzyme
Methylated cytidine plays an important role as an epigenetic signal in gene regulation. Its oxidation products are assumed to be involved in active demethylation processes but also in damaging DNA. Here, we report the photochemical production of the 5‐methyl‐2′‐deoxycytidine radical cation via a two‐photon ionization process. The radical cation is detected by time‐resolved IR spectroscopy and identified by band assignment using density functional theory calculations. Two final oxidation products are characterized with liquid chromatography coupled to mass spectrometry.