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Multi‐Color Imaging of Fluorescent Nanodiamonds in Living HeLa Cells Using Direct Electron‐Beam Excitation
Author(s) -
Nawa Yasunori,
Inami Wataru,
Lin Sheng,
Kawata Yoshimasa,
Terakawa Susumu,
Fang ChiaYi,
Chang HuanCheng
Publication year - 2014
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201300802
Subject(s) - fluorescence , excitation , hela , cathode ray , materials science , chemistry , optics , optoelectronics , electron , photochemistry , physics , biochemistry , quantum mechanics , cell
Abstract Multi‐color, high spatial resolution imaging of fluorescent nanodiamonds (FNDs) in living HeLa cells has been performed with a direct electron‐beam excitation‐assisted fluorescence (D‐EXA) microscope. In this technique, fluorescent materials are directly excited with a focused electron beam and the resulting cathodoluminescence (CL) is detected with nanoscale resolution. Green‐ and red‐light‐emitting FNDs were employed for two‐color imaging, which were observed simultaneously in the cells with high spatial resolution. This technique could be applied generally for multi‐color immunostaining to reveal various cell functions.