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Isolation and Enrichment of Pathogens with a Surface‐Modified Aluminium Chip for Raman Spectroscopic Applications
Author(s) -
Pahlow Susanne,
Kloß Sandra,
Blättel Verena,
Kirsch Konstantin,
Hübner Uwe,
Cialla Dana,
Rösch Petra,
Weber Karina,
Popp Jürgen
Publication year - 2013
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201300543
Subject(s) - aluminium , raman spectroscopy , isolation (microbiology) , materials science , nanotechnology , surface enhanced raman spectroscopy , chip , chemistry , raman scattering , metallurgy , microbiology and biotechnology , optics , biology , physics , electrical engineering , engineering
We developed a Raman‐compatible chip for isolating microorganisms from complex media. The isolation of bacteria is achieved by using antibodies as capture molecules. Due to the very specific interaction with the targets, this approach is promising for isolation of bacteria even from complex matrices such as body fluids. Our choice of capture molecules also enabled the investigation of samples containing yet unidentified bacteria, as the antibodies can capture a large variety of bacteria based on their analogue cell wall surface structures. The capability of our system is demonstrated for a broad range of different Gram‐positive and Gram‐negative germs. Subsequent identification is done by recording Raman spectra of the bacteria. Further, it is shown that classification with chemometric methods is possible.