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A His‐Tagged Melanocarpus albomyces Laccase and its Electrochemistry upon Immobilisation on NTA‐Modified Electrodes and in Conducting Polymer Films
Author(s) -
Sosna Maciej,
Boer Harry,
Bartlett Philip N.
Publication year - 2013
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201300340
Subject(s) - laccase , nitrilotriacetic acid , glassy carbon , chemistry , electrochemistry , cyclic voltammetry , electrode , redox , electron transfer , polymer , biosensor , aniline , conductive polymer , immobilized enzyme , combinatorial chemistry , inorganic chemistry , polymer chemistry , organic chemistry , enzyme , biochemistry , chelation
Abstract The article describes the construction, immobilisation and electrochemistry of histidine tagged laccase from Melanocarpus albomyces . A facile method of functionalisation of glassy carbon electrodes with nitrilotriacetic acid (NTA) using diazonium grafting and solid state chemistry is described. NTA‐modified electrodes are shown to bind laccase which reduces oxygen at neutral pH in the presence of soluble redox mediator. Laccase‐modified electrodes are also prepared by enzyme immobilisation within poly(aniline)/poly(vinylsulfonate) films. The polymer is found to efficiently retain the enzyme as well as provide direct electrical contact between the electrode and the enzyme active centre. Cyclic voltammetry reveals the direct electron transfer to the enzyme is dependent on the redox state of the polymer film.