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Fast Real‐Time NMR Methods for Characterizing Short‐Lived Molecular States
Author(s) -
Rennella Enrico,
Brutscher Bernhard
Publication year - 2013
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201300339
Subject(s) - molecular dynamics , nuclear magnetic resonance spectroscopy , chemistry , characterization (materials science) , chemical physics , biological system , nanotechnology , computational chemistry , materials science , biology , organic chemistry
The characterization of both the structure and the conformational dynamics of biological macromolecules, namely proteins and nucleic acids, is required for understanding the molecular mechanisms underlying physiological function and disease. Molecular dynamics involves the transient departure from the ground‐state structures to populate short‐lived excited state conformations that can play important functional roles. Real‐time multi‐dimensional NMR spectroscopy represents a unique tool for investigating dynamic molecular processes occurring on time scales of seconds or longer, providing atomic‐resolution information about short‐lived states. In this minireview, we discuss recent progress made in the field of fast real‐time multidimensional NMR. The potential of these new methods is illustrated for several biomolecular systems that have recently been studied by fast real‐time multidimensional NMR.