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Extracellular Electron Transfer across Bacterial Cell Membranes via a Cytocompatible Redox‐Active Polymer
Author(s) -
Nishio Koichi,
Nakamura Ryuhei,
Lin Xiaojie,
Konno Tomohiro,
Ishihara Kazuhiko,
Nakanishi Shuji,
Hashimoto Kazuhito
Publication year - 2013
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201300117
Subject(s) - phosphorylcholine , chemistry , electron transfer , membrane , phospholipid , extracellular , biophysics , redox , bacterial cell structure , viability assay , electron transport chain , bacteria , biochemistry , cell , photochemistry , biology , organic chemistry , genetics
A redox‐active phospholipid polymer with a phospholipid‐mimicking structure (2‐methacryloyloxyethyl phosphorylcholine; MPC) was synthesized to construct a biocompatible electron mediator between bacteria and an electrode. In this study, a copolymer of MPC and vinylferrocene [VF; poly(MPC‐ co ‐VF)] (PMF) is synthesized. When PMF is added to cultures of the bacterial species Escherichia coli (Gram negative) and Lactobacillus plantarum (Gram positive), which have different cell wall structures, a catalytic current mediated by PMF is observed. In addition, growth curves and live/dead assays indicate that PMF does not decrease metabolic activity or cell viability. These results indicate that PMF mediates extracellular electron transfer across bacterial cell membranes without associated cytotoxicity.