Premium
Optimization of a Membraneless Glucose/Oxygen Enzymatic Fuel Cell Based on a Bioanode with High Coulombic Efficiency and Current Density
Author(s) -
Shao Minling,
Zafar Muhammad Nadeem,
Falk Magnus,
Ludwig Roland,
Sygmund Christoph,
Peterbauer Clemens K.,
Guschin Dmitrii A.,
MacAodha Domhnall,
Ó Conghaile Peter,
Leech Dónal,
Toscano Miguel D.,
Shleev Sergey,
Schuhmann Wolfgang,
Gorton Lo
Publication year - 2013
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201300046
Subject(s) - cellobiose dehydrogenase , faraday efficiency , bilirubin oxidase , cellobiose , chemistry , anode , chemical engineering , biochemistry , electrode , cellulose , cellulase , enzyme , engineering
After initial testing and optimization of anode biocatalysts, a membraneless glucose/oxygen enzymatic biofuel cell possessing high coulombic efficiency and power output was fabricated and characterized. Two sugar oxidizing enzymes, namely, pyranose dehydrogenase from Agaricus meleagris ( Am PDH) and flavodehydrogenase domains of various cellobiose dehydrogenases (DH CDH ) were tested during the pre‐screening. The enzymes were mixed, “wired” and entrapped in a low‐potential Os‐complex‐modified redox‐polymer hydrogel immobilized on graphite. This anode was used in combination with a cathode based on bilirubin oxidase from Myrothecium verrucaria adsorbed on graphite. Optimization showed that the current density for the mixed enzyme electrode could be further improved by using a genetically engineered variant of the non‐glycosylated flavodehydrogenase domain of cellobiose dehydrogenase from Corynascus thermophilus expressed in E. coli (ngDH Ct CDHC310Y ) with a high glucose‐turnover rate in combination with an Os‐complex‐modified redox polymer with a high concentration of Os complexes as well as a low‐density graphite electrode. The optimized biofuel cell with the Am PDH/ngDH Ct CDHC310Y anode showed not only a similar maximum voltage as with the biofuel cell based only on the ngDH Ct CDHC310Y anode (0.55 V) but also a substantially improved maximum power output (20 μW cm −2 ) at 300 mV cell voltage in air‐saturated physiological buffer. Most importantly, the estimated half‐life of the mixed biofuel cell can reach up to 12 h, which is apparently longer than that of a biofuel cell in which the bioanode is based on only one single enzyme.