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A Monolayer Assay Tailored to Investigate Lipid–Protein Systems
Author(s) -
Chwastek Grzegorz,
Schwille Petra
Publication year - 2013
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201300035
Subject(s) - monolayer , membrane , fluorescence , fluorescence microscope , nanotechnology , confocal microscopy , chemistry , fluorescence correlation spectroscopy , fluorescence spectroscopy , microscopy , biophysics , materials science , biochemistry , molecule , biology , physics , microbiology and biotechnology , organic chemistry , optics , quantum mechanics
Model membrane systems have become invaluable tools to investigate specific features of cellular membranes. Although a variety of different experimental assays does exist, many of them are rather complicated in their preparation and difficult in their practical realisation. Here, we propose a new simple miniaturised monolayer assay that can easily be combined with standard analytical techniques such as confocal fluorescence microscopy and fluorescence correlation spectroscopy (FCS).