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Photoelectrochemical Sensor Based on Quantum Dots and Sarcosine Oxidase
Author(s) -
Riedel Marc,
Göbel Gero,
Abdelmonem Abuelmagd M.,
Parak Wolfgang J.,
Lisdat Fred
Publication year - 2013
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201201036
Subject(s) - sarcosine , photocurrent , surface plasmon resonance , polyelectrolyte , chemistry , allylamine , quantum dot , analytical chemistry (journal) , materials science , nanotechnology , optoelectronics , organic chemistry , glycine , biochemistry , amino acid , nanoparticle , polymer
In this study, a photobioelectrochemical sensor for the detection of sarcosine is reported. For this purpose, CdSe/ZnS quantum dot (QD) modified electrodes are prepared and the oxygen‐dependent photocurrent is evaluated under illumination. By using sarcosine oxidase (SOD), the photocurrent can be suppressed because of biocatalytic oxygen reduction. For the construction of a sensor, SOD is immobilised on the QDs by means of the polyelectrolyte poly(allylamine hydrochloride) (PAH). Multi‐layer systems have been built up to six bilayers through electrostatic interactions. The assembly can be verified by surface plasmon resonance measurements. By varying the number of layers, the influence of the amount of enzyme on the sensitivity of the sensor can be shown. The [SOD/PAH] 6 ‐layer system results in a signal change of 0.041 % μ M −1 in the linear range from 100 μ M to 1 m M of sarcosine.