Premium
Physicochemical Characterization of Acidiphilium sp. Biofilms
Author(s) -
Malki Moustafa,
Casado Santiago,
López María Francisca,
Caillard Renaud,
Palomares F. Javier,
Gago José A. Martín,
VazDomínguez Cristina,
Cuesta Angel,
Amils Ricardo,
Fernández Víctor M.,
Velez Marisela,
De Lacey Antonio L.,
Olea David
Publication year - 2013
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201201034
Subject(s) - biofilm , x ray photoelectron spectroscopy , extracellular polymeric substance , chemistry , substrate (aquarium) , chemical engineering , fluorescence microscope , analytical chemistry (journal) , bacteria , fluorescence , chromatography , biology , genetics , physics , quantum mechanics , engineering , ecology
Abstract The biofilm formation of a strain of the extremophile bacterium Acidiphilium sp., capable of donating electrons directly to electrodes, was studied by different surface characterization techniques. We develop a method that allows the simultaneous study of bacterial biofilms by means of fluorescence microscopy and atomic force microscopy (AFM), in which transparent graphitic flakes deposited on a glass substrate are used as a support for the biofilm. The majority of the cells present on the surface were viable, and the growth of the biofilms over time showed a critical increase of the extracellular polymeric substances (EPS) as well as the formation of nanosized particles inside the biofilm. Also, the presence of Fe in Acidiphilium biofilms was determined by X‐ray photoelectron spectroscopy (XPS), whereas surface‐enhanced infrared absorption spectroscopy indicated the presence of redox‐active proteins.