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Two‐Photon Absorption and Time‐Resolved Stimulated Emission Depletion Spectroscopy of a New Fluorenyl Derivative
Author(s) -
Belfield Kevin D.,
Bondar Mykhailo V.,
Morales Alma R.,
Yue Xiling,
Luchita Gheorghe,
Przhonska Olga V.,
Kachkovsky Olexy D.
Publication year - 2012
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201200405
Subject(s) - fluorescence , time resolved spectroscopy , femtosecond , emission spectrum , absorption (acoustics) , spectroscopy , analytical chemistry (journal) , absorption spectroscopy , laser induced fluorescence , chemistry , fluorescence spectroscopy , photochemistry , two photon absorption , fluorene , materials science , spectral line , laser , optics , organic chemistry , physics , quantum mechanics , composite material , polymer , astronomy
The synthesis, comprehensive linear photophysical characterization, two‐photon absorption (2PA), steady‐state and time‐resolved stimulated emission depletion properties of a new fluorene derivative, ( E )‐1‐(2‐(di‐ p ‐tolylamino)‐9,9‐diethyl‐9 H ‐fluoren‐7‐yl)‐3‐(thiophen‐2‐yl)prop‐2‐en‐1‐one ( 1 ), are reported. The primary linear spectral properties, including excitation anisotropy, fluorescence lifetimes, and photostability, were investigated in a number of aprotic solvents at room temperature. The degenerate 2PA spectra of 1 were obtained with open‐aperture Z‐scan and two‐photon induced fluorescence methods, using a 1 kHz femtosecond laser system, and maximum 2PA cross‐sections of ∼400–600 GM were obtained. The nature of the electronic absorption processes in 1 was investigated by DFT‐based quantum chemical methods implemented in the Gaussian 09 program. The one‐ and two‐photon stimulated emission spectra of 1 were measured over a broad spectral range using a femtosecond pump–probe‐based fluorescence quenching technique, while a new methodology for time‐resolved fluorescence emission spectroscopy is proposed. An effective application of 1 in fluorescence bioimaging was demonstrated by means of one‐ and two‐photon fluorescence microscopy images of HCT 116 cells containing dye encapsulated micelles.