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Enzymatic Reaction in Droplets Manipulated with Liquid Dielectrophoresis
Author(s) -
Kumemura Momoko,
Collard Dominique,
Yoshizawa Satoko,
Wee Bernard,
Takeuchi Shoji,
Fujita Hiroyuki
Publication year - 2012
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201200354
Subject(s) - dielectrophoresis , joule heating , nanotechnology , chemistry , microfluidics , materials science , composite material
Droplet generation and transportation for biological reactions are conducted with liquid dielectrophoresis (LDEP), forming two hundred picoliter droplets and aligning them in an open environment above the micro‐machined electrodes. The generation of the dielectrophoresis signals was critically examined to actuate droplets in biological solutions without excessive Joule heating. Enzymatic reactions between β‐galactosidase and fluorescein di‐β‐ D ‐galactopyranoside were succeeded in manipulated droplets, which was confirmed by fluorescence imaging. These results allow us to propose the integration of LDEP actuation in high throughput biomolecular assays.