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Cover Picture: Improved Temporal Resolution and Linked Hidden Markov Modeling for Switchable Single‐Molecule FRET (ChemPhysChem 3/2011)
Author(s) -
Uphoff Stephan,
Gryte Kristofer,
Evans Geraint,
Kapanidis Achillefs N.
Publication year - 2011
Publication title -
chemphyschem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201190011
Subject(s) - förster resonance energy transfer , single molecule fret , chemistry , molecule , acceptor , markov chain , stoichiometry , cover (algebra) , chemical physics , nanotechnology , fluorescence , biological system , materials science , computer science , physics , organic chemistry , machine learning , biology , condensed matter physics , mechanical engineering , quantum mechanics , engineering
Theodor Förster, whose 100th birthday is celebrated in this special issue, first described and formalized Förster resonance energy transfer (FRET). FRET as a method provides unique information on biomolecular structure and dynamics, especially at the single‐molecule level. The cover picture illustrates a recent extension to the single‐molecule toolbox. Switchable FRET monitors multiple FRET pairs on the same molecule sequentially by switching acceptor fluorophores on and off. The two FRET pairs on one DNA molecule appear as distinct populations in a plot of FRET and donor–acceptor stoichiometry data. Optimized conditions as described by A. N. Kapanidis et al. on p. 571 improve the temporal resolution of the method by an order of magnitude. Furthermore, linked hidden Markov modeling analysis identifies transient FRET states and links them to the donor–acceptor stoichiometry.