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Intracellular Conformations of Human Telomeric Quadruplexes Studied by Electron Paramagnetic Resonance Spectroscopy
Author(s) -
Azarkh Mykhailo,
Singh Vijay,
Okle Oliver,
Dietrich Daniel R.,
Hartig Jörg S.,
Drescher Malte
Publication year - 2012
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201100980
Subject(s) - electron paramagnetic resonance spectroscopy , electron paramagnetic resonance , spectroscopy , intracellular , chemistry , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , resonance (particle physics) , crystallography , biophysics , stereochemistry , biochemistry , biology , physics , atomic physics , quantum mechanics
In‐cell DEER ( in‐cell double electron electron resonance) is applied to investigate quadruplex formation of the human telomeric repeat d[AGGG(TTAGGG) 3 ]. The initially unfolded DNA sequence forms a mixture of different quadruplex topologies upon injection into living cells. In addition, time‐dependent distance measurements are carried out to monitor quadruplex folding.