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A Protein Biosensor That Relies on Bending of Single DNA Molecules
Author(s) -
Crawford Robert,
Kelly Douglas J.,
Kapanidis Achillefs N.
Publication year - 2012
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201100881
Subject(s) - dna , biosensor , activator (genetics) , biophysics , chemistry , molecule , dna binding protein , microbiology and biotechnology , biochemistry , nanotechnology , biology , gene , materials science , transcription factor , organic chemistry
A “bendy” protein sensor: A DNA‐based sensor that uses folded DNA (through DNA kinks) and protein‐induced bending to detect DNA‐binding proteins is presented. Single‐molecule sensing of a transcriptional activator (catabolite activator protein, CAP, which bends its DNA site by 80°) is demonstrated in solution and on surfaces, both in buffers and in cell lysates. The method should allow detection of a wide range of DNA‐bending proteins.