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Single‐Molecule DNA Force Spectroscopy to Probe Interactions with the Tri‐Peptide Lys‐Trp‐Lys
Author(s) -
Murade Chandrashekhar U.,
Subramaniam Vinod,
Otto Cees,
Bennink Martin L.
Publication year - 2011
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201100561
Subject(s) - force spectroscopy , molecule , chemistry , optical tweezers , spectroscopy , magnetic tweezers , dna , molecular tweezers , peptide , crystallography , stereochemistry , supramolecular chemistry , biochemistry , organic chemistry , physics , quantum mechanics
May the force be with you: It is shown that single‐molecule optical tweezers force spectroscopy can be used successfully to detect the effects of binding of low‐affinity ligands to a single DNA molecule (see picture) and to determine various kinetic parameters of the reaction at single‐molecular level.