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Improved Photon Yield from a Green Dye with a Reducing and Oxidizing System
Author(s) -
Le Gall Antoine,
Dulin David,
Clavier Gilles,
MéalletRenault Rachel,
Bouyer Philippe,
Perronet Karen,
Westbrook Nathalie
Publication year - 2011
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201100085
Subject(s) - oxidizing agent , photobleaching , fluorophore , photochemistry , bodipy , chemistry , yield (engineering) , boron , oxygen , steric effects , fluorescence , materials science , organic chemistry , optics , physics , metallurgy
An optimized chemistry turns a small green dye into a useful label for single‐molecule experiments where steric hindrance is an issue. Thanks to a reducing and oxidizing system combined to oxygen depletion, a single Bodipy (boron‐dipyrromethene) FL fluorophore emits, on average, 20 times more photons around 510 nm (see picture) and its lifetime before photobleaching is increased by the same amount, reaching several seconds.