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FRET Imaging by k t / k f
Author(s) -
Roberti M. Julia,
Giordano Luciana,
Jovin Thomas M.,
JaresErijman Elizabeth A.
Publication year - 2011
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201000925
Subject(s) - förster resonance energy transfer , excited state , chemistry , reaction rate constant , acceptor , analytical chemistry (journal) , atomic physics , fluorescence , kinetics , physics , optics , quantum mechanics , chromatography , condensed matter physics
A FRET formalism in terms of the ratio between the rate constants for deactivation of the donor excited state by resonance energy transfer ( k t ) and by emission ( k f ) is introduced (see picture). The advantages of the approach are illustrated by FRET imaging of the donor/acceptor system consisting of co‐aggregates of the amyloid protein alpha‐synuclein labeled with the biarsenical FlAsH and quantum dots (605 nm) functionalized with alpha‐synuclein.