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FRET in Cell Biology: Still Shining in the Age of Super‐Resolution?
Author(s) -
Grecco Hernán E.,
Verveer Peter J.
Publication year - 2011
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201000795
Subject(s) - förster resonance energy transfer , resolution (logic) , microscopy , nanotechnology , superresolution , biological imaging , materials science , physics , optics , computer science , fluorescence , artificial intelligence , image (mathematics)
Interest in imaging of Förster resonance energy transfer (FRET) in biological systems has been steadily increasing in the last 30 years. The ability to transduce a near‐field interaction into a far‐field signal has provided a unique optical tool to assess biological phenomena well below the resolution of standard optical microscopy. In recent years, sub‐diffraction microscopy techniques have achieved maturation and are increasingly used in biological applications. As the resolution of these methods increases they will slowly encroach on the domains where FRET is now dominant. Herein we review the major applications in biological FRET imaging and we discuss the possibilities and challenges in the super‐resolution era.