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Simultaneous Optical and Electrical Recording of Single Molecule Bonding to Single Channel Proteins
Author(s) -
Ide Toru
Publication year - 2010
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201000560
Subject(s) - total internal reflection fluorescence microscope , molecule , channel (broadcasting) , optical recording , bilayer , single molecule experiment , reflection (computer programming) , fluorescence , microscope , chemistry , nanotechnology , computer science , materials science , optoelectronics , optics , physics , telecommunications , membrane , organic chemistry , biochemistry , programming language
Two birds with one stone: A method is described for simultaneous optical and electrical recordings of single ion‐channel proteins by combining the artificial bilayer technique and single‐molecule imaging with a total internal reflection fluorescence microscope. Using this apparatus, interactions between single DNA molecules and single alpha‐hemolysin channels are measured (see picture).