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Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells
Author(s) -
Shin Young Shik,
Ahmad Habib,
Shi Qihui,
Kim Hyungjun,
Pascal Tod A.,
Fan Rong,
Goddard William A.,
Heath James R.
Publication year - 2010
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201000528
Subject(s) - boulevard , library science , multiplex , nanotechnology , engineering , biology , geography , archaeology , computer science , bioinformatics , materials science
The optimization of chemistries to enable the patterning of miniaturized DNA barcodes using microfluidics flow channels is described (see picture). Experiment and theory reveal that solvent mixtures in which counterions are strongly associated with the negatively charged DNA oligomers may be harnessed to produce high quality, high density DNA microarray patterns over a large area.