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Mechanism of Proton Relaxation for Enzyme‐Manipulated, Multicomponent Gold–Magnetic Nanoparticle Chains
Author(s) -
Jaganathan Hamsa,
Gieseck Richard L.,
Hudson Katherine,
Kellogg Michael,
Ramaswamy Aneesh K.,
Raver Kimberly E.,
Smith Tyler,
Vacchiano Ashley N.,
Wager Andrew,
Ivanisevic Albena
Publication year - 2010
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201000397
Subject(s) - relaxation (psychology) , colloidal gold , superparamagnetism , nanoparticle , chemistry , iron oxide nanoparticles , magnetization , materials science , nuclear magnetic resonance , nanotechnology , magnetic field , psychology , social psychology , physics , quantum mechanics
Longitudinal and transverse relaxation times of multicomponent nanoparticle (NP) chains are investigated for their potential use as multifunctional imaging agents in magnetic resonance imaging (MRI). Gold NPs (ca. 5 nm) are arranged linearly along double‐stranded DNA, creating gold NP chains. After cutting gold NP chains with restriction enzymes (EcoRI or BamHI), multicomponent NP chains are formed through a ligation reaction with enzyme‐cut, superparamagnetic NP chains. We evaluate the changes in relaxation times for different constructs of gold–iron oxide NP chains and gold–cobalt iron oxide NP chains using 300 MHz 1 H NMR. In addition, the mechanism of proton relaxation for multicomponent NP chains is examined. The results indicate that relaxation times are dependent on the one‐dimensional structure and the amount of superparamagnetic NP chains present in the multicomponent constructs. Multicomponent NP chains arranged on double‐stranded DNA provide a feasible method for fabrication of multifunctional imaging agents that improve relaxation times effectively for MRI applications.