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The Influence of Surface Composition of Nanoparticles on their Interactions with Serum Albumin
Author(s) -
Treuel Lennart,
Malissek Marcelina,
Gebauer Julia Susanne,
Zellner Reinhard
Publication year - 2010
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.201000174
Subject(s) - circular dichroism , bovine serum albumin , adsorption , nanoparticle , chemistry , silver nanoparticle , protein adsorption , polystyrene , polymer , colloidal gold , chemical engineering , metal , serum albumin , crystallography , chromatography , organic chemistry , biochemistry , engineering
Interactions between differently functionalised silver and gold nanoparticles (NPs) as well as polystyrene nanoparticles with bovine serum albumin (BSA) are studied using circular dichroism (CD) spectroscopy. It is found that the addition of NPs to the protein solution destroys part of the helical secondary structure of the protein as a result of surface adsorption. From the loss of free protein and hence the extent of their structural change adsorption equilibrium constants are derived. The results reveal that citrate‐coated gold and silver NPs exhibit much stronger interactions with BSA than polymeric or polymer‐coated metallic NPs. It is therefore concluded that for the particles considered, the influence of surface composition on the interaction behaviour dominates that of the core.