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The “Wiggling and Jiggling of Atoms” Leading to Excited‐State Proton Transfer in Green Fluorescent Protein
Author(s) -
Nienhaus G. Ulrich
Publication year - 2010
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200901016
Subject(s) - excited state , chromophore , femtosecond , deprotonation , photochemistry , fluorescence , femtochemistry , green fluorescent protein , raman spectroscopy , chemistry , spectroscopy , proton , atomic physics , optics , laser , ion , physics , biochemistry , quantum mechanics , gene , organic chemistry
Shaking off the blues: Femtosecond stimulated Raman spectroscopy reveals vibrational dynamics accompanying excited state deprotonation of the GFP chromophore, which causes a color change from blue to green (see picture).