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High‐Quality Covalently Grafting Hemoglobin on Gold Electrodes: Characterization, Redox Thermodynamics and Bio‐electrocatalysis
Author(s) -
Tian Yuan,
Ran Qin,
Xu Jingjing,
Xian Yuezhong,
Peng Ru,
Jin Litong
Publication year - 2009
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200900588
Subject(s) - monolayer , covalent bond , chemistry , redox , electrocatalyst , cyclic voltammetry , biosensor , biocompatibility , infrared spectroscopy , self assembled monolayer , ligand (biochemistry) , click chemistry , photochemistry , electrochemistry , electrode , inorganic chemistry , combinatorial chemistry , organic chemistry , biochemistry , receptor
Abstract Herein, we report a versatile surface chemistry methodology to covalently immobilize ligands and proteins to self‐assembled monolayers (SAMs) on gold electrode. The strategy is based on two steps: 1) the coupling of soluble azido‐PEG‐amimo ligand with an alkynyl‐terminated monolayer via click reaction and 2) covalent immobilization hemoglobin (Hb) to the amine‐terminated ligand via carbodiimide reaction. Surface‐enhanced Raman scattering spectroscopy (SERS), atomic force microscopy (AFM), reflection absorption infrared spectroscopy (RAIR) and cyclic voltammetry are used to characterize the model interfacial reactions. We also demonstrate the excellent biocompatibility of the interface for Hb immobilization and reliable application of the proposed method for H 2 O 2 biosensing. Moreover, the redox thermodynamics of the Fe 3+ /Fe 2+ couple in Hb is also investigated.