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A Force‐Spectroscopy‐Based Single‐Molecule Metal‐Binding Assay
Author(s) -
Cao Yi,
Er Kai Shih,
Parhar Rakesh,
Li Hongbin
Publication year - 2009
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200900333
Subject(s) - force spectroscopy , molecule , chemistry , metal , spectroscopy , metal ions in aqueous solution , crystallography , analytical chemistry (journal) , computational chemistry , organic chemistry , physics , quantum mechanics
Quantifying metal‐binding by force: A quantitative single‐molecule force‐spectroscopy‐based assay is developed to measure the binding affinity of metal ions to proteins. The method uses the unfolding force of a protein as a direct probe to distinguish the apo and metal‐ion‐bound forms of that protein and quantify the partitioning between the two forms (see figure).